SL NO |
NAME OF THE TEST |
REFERENCE RANGE |
Methodology |
Sample |
Volume of sample to be collected & instruction for collection |
Sample handling |
Processing schedule |
Turnaround time (TAT) |
TAT for urgent tests |
Clinical utility &Information needed |
1 |
FBS |
Child |
60-100mg/dl |
Hexokinase/G-6-PDH |
Serum |
2ml Blood in plain tube, ≥ 8 hours of no calorie intake |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Diabetesmellitus, impaired glucosetoleranceandhypoglycaemic conditions |
Adult |
74 -106 mg/dl |
Hexokinase/G-6-PDH |
2 |
PPBS |
|
< 140 mg/dl |
Hexokinase/G-6-PDH |
Serum |
2ml Blood in plain tube, 2 hours after calorie intake |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Diabetesmellitus, impaired glucosetoleranceandhypoglycaemic conditions |
3 |
RBS |
|
70 - 140 mg/dl |
Hexokinase/G-6-PDH |
Serum |
2ml Blood in plain tube |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Diabetesmellitus, impaired glucosetoleranceandhypoglycaemic conditions |
4 |
GCT |
|
< 140 mg/dl |
Hexokinase/G-6-PDH |
Serum |
2ml Blood in plain tube . (75 g glucose, after 2 hr) |
Regular |
24 *7 |
Same day after 2 hours of lastsample |
2 to 4 hour |
Diagnosis of gestational diabetes mellitus |
5 |
GTT |
|
|
Hexokinase/G-6-PDH |
Serum & Urine |
2ml Blood in plain tube . (FPG,75g) glucose after 30 min, 1hr,1.30 hr, 2 hr) (GTT after GCT-FPG, 100 g glucose, 1hr,2 hr, 3 hr) |
Regular |
24 *7 |
Same day after 2 hours of lastsample |
2 to 4 hour |
Diagnosis of Diabetes mellitus, Gestational DM, Renalglycosuria. |
6 |
HbA1C |
|
4.5 - 5.7% |
Enzymatic |
Whole Blood |
2ml Whole Blood in EDTA tube. |
Regular |
24 *7 |
Same day after 2hrs. |
2 to 4 hour |
Diagnosis and management of diabetes mellitus |
|
KIDNEY PROFILE |
|
|
|
|
|
|
|
|
|
|
7 |
UREA |
|
15 - 43 mg/dl |
Urease |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 |
2 to 4 hour |
Kidney function test |
8 |
CREATININE |
|
0.5 - 1.2 mg/dl |
Alkaline Picrate |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hrs |
1 hour |
Renal function test |
|
LIPID PROFILE |
|
|
|
Serum |
3 ml Blood in Plain tube (12 hour fasting sample) |
Regular |
24 *7 |
Same day after 2hours |
1hour |
Hypolipidemia and Hyperlipidemia. |
8 |
TOTAL CHOLESTEROL |
|
Upto 200mg/dl |
Enzymatic |
Serum |
3 ml Blood in Plain tube (fasting sample) |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Increasedlevelsrelated to increased risk of cardiovascular disease. |
9 |
HDL |
|
40-60 mg/dl |
Accelerator Selective Detergent |
Serum |
3 ml Blood in Plain tube (fasting sample) |
Regular |
24*7 |
Same day after 2 hours |
1 hour |
Risk stratification of IHD |
10 |
Direct LDL |
Optimal |
<100 mg/dl |
Liquid selective Detergent |
Serum |
3 ml Blood in Plain tube (fasting sample) |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Risk stratification of IHD |
Near optimal |
100-129mg/dl |
Borderline high |
130-159 mg/dl |
High |
160-189mg/dl |
Very High |
>190 |
11 |
TRIGLYCERIDES |
|
Upto 150mg/dl |
Glycerol phosphate oxiadase |
Serum |
3 ml Blood in Plain tube (fasting sample) |
Regular |
24*7 |
Same day after 2 hours |
1 hour |
Risk stratification of IHD |
12 |
VLDL |
|
Upto 40mg/dl |
Calculated |
Serum |
3 ml Blood in Plain tube (fasting sample) |
Regular |
24*7 |
Same day after 2 hours |
1 hour |
Risk stratification of IHD |
|
LIVER FUNCTION TESTS |
|
|
|
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Liver function assessment |
13 |
TOTAL BILIRUBIN |
|
0.2 - 1.2 mg/dl |
Diazonium Salt |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Used for patients who show signs of abnormal liver function. |
14 |
DIRECT BILIRUBIN |
|
Upto 0.5 mg/dl |
Diazo Reaction |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Used for patients who show signs of abnormal liver function. |
15 |
INDIRECT BILIRUBIN |
|
|
Calculated |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Used for patients who show signs of abnormal liver function., andhaemolyticcausesof jaundice |
16 |
TOTAL PROTEIN |
|
6.4 - 8.3 g/dl |
Biuret reaction, End point |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Used for determining if patient has liver disease or kidney disease. |
17 |
ALBUMIN |
|
3.5 - 5.2 g/dl |
Bromcresol green |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Used for determining if patient has liver disease or kidney disease. |
18 |
GLOBULIN |
|
1.8 - 3.4 g/dl |
Calculated |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hrs |
1 hour |
Increasedlevelrelated to chronic infections, Hepatic diseases,multiplemyeloma |
19 |
AG RATIO |
|
|
Calculated |
Serum |
3 ml Blood in Plain |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Altered in Liver disease, Renal disease, Malnutrition and Malabsorption |
20 |
ALKALINE PHOSPHATASE |
|
40-150 U/L |
AMP optimized to IFCC |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Majority of ALP activity is derived from the liver and bone. Concentrations are increased inpatients with biliary obstructive disorders, tumor of liver and bone etc. |
21 |
AST(SGOT) |
|
5-34 U/L |
TRIS buffer without P5P |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2 hours |
1 hour |
Obstructive liver disease, liver function assessment |
22 |
ALT(SGPT) |
|
0-55 U/L |
TRIS buffer with P5P |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Liver cell injury, liver function assessment |
|
CARDIAC PROFILE |
|
|
|
|
|
|
|
|
|
|
23 |
CKMB- Activity |
|
5-25 IU/L |
IFCC/Immunologic UV |
Serum |
3 ml Blood in Plain tube |
Transport ASAP |
24*7 |
Same day after 2hours |
1 hour |
Myocardial Infarction |
24 |
CK TOTAL |
|
22 -198 U/L |
IFCC |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Myocardial, Cerebro- vascular, skeletal muscular disease |
25 |
hs TROPONIN I |
Male |
0-34.2pg/ml |
CMIA |
serum |
2 ml Blood in plain tube |
Transport ASAP |
24*7 |
Same day after 2 hour |
2 to 4 hour |
Marker of Myocardial Infarction |
Female |
0-15.6pg/ml |
|
ELECTROLYTES |
|
|
|
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 hours |
1 hour |
Useful in assessing acid base balance and electrolyte balance in a variety of medicalconditions. |
26 |
SODIUM |
|
135-145 mEq/L |
ISE |
serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Analysis of Hyper or Hyponatraemia |
27 |
POTASSIUM |
|
3.5-5.0 mEq/L |
ISE |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Differential diagnosis of hypokalemia and hyperkalemia, metabolic acidosis,metabolic alkalosis |
28 |
CHLORIDE |
|
96-106 mEq/L |
ISE |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Electrolyte imbalance |
|
THYROID FUNCTION TESTS |
|
|
|
|
|
|
|
|
|
29 |
TSH |
Children |
0.7-6.4 µIU/ml |
CMIA |
Serum |
3 ml Blood in Plain tube. |
Regular |
24*7 |
Same day after 2 to 4 hours |
2 to 4 hour |
Thyroid function test |
Adult |
0.4 - 4.2 µIU/ml |
30 |
FREE T3 |
|
1.71- 3.71 pg/ml |
CMIA |
Serum |
3 ml Blood in Plain tube. |
Regular |
24*7 |
Same day after 2 to 4 hours |
2 to 4 hour |
Thyroid function test |
31 |
FREE T4 |
|
0.70 - 1.48 ng/dl |
CMIA |
Serum |
3 ml Blood in Plain tube. |
Regular |
24*7 |
Same day after 2 to 4 hours |
2 to 4 hour |
Thyroid function test |
|
OTHERS |
|
|
|
|
|
|
|
|
|
|
32 |
PHOSPHOROUS |
|
2.5-4.5 mg/dl |
Phospho molybdate UV |
serum |
2 ml Blood in plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
To evaluate the level of phosphate in your blood |
33 |
URIC ACID |
|
3.5-7.2 mg/dl |
Uricase Enzymatic |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 |
2 to 4 hour |
Marker of increased nucleic acid turnover and gout |
34 |
TOTAL CALCIUM |
|
8.4-10.2 mg/dl |
Arsenazo dye method |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 to 4 hours |
1 hour |
High level in Hyperparathyroidism, Malignancy, Sarcoidosis.Lowlevels inHypoalbuminemia, renal insufficiency, Hypoparathyroidism. |
35 |
CHOLINESTERASE |
|
4850-12000 U/L |
Butrylcholinesterase method |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hours |
1 hour |
Marker of organophosphorus poisoning |
36 |
LDH |
|
125-220U/L |
IFCC |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Marker enzyme for cell turnover, cell destruction; increased in haemolytic diseaseas,liverdiseases, etc... |
37 |
GGT |
Male |
<55U/L |
L-Gamma glutamyl-3-carboxy-4-Nitro anilide |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2hrs |
1 hour |
Alcoholic liver disease and cholestasis |
Female |
<38U/L |
38 |
MAGNESIUM |
Newborn, 2-4 d |
1.5-2.2 mg/dL |
Enzymatic |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2 hours |
1 hour |
Assessment of hypo &hypermagnesemia |
5 mo-6 y |
1.7-2.3 mg/dL |
6-12 y |
1.7-2.1 mg/dL |
>12 y |
1.6-2.6 mg/dL |
39 |
HOMOCYSTEINE |
With Folate Supplement |
|
CMIA |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hour |
2 to 4 hour |
Assessment of Iron deficiency and Iron Overload |
<15 yrs |
< 8 μmol/L |
15-65 yrs |
< 12 μmol/L |
>65 yrs |
< 16 μmol/L |
No Folate Supplement |
|
<15 yrs |
< 10 μmol/L |
15-65 yrs |
< 15 μmol/L |
>65 yrs |
< 20 μmol/L |
|
HORMONES |
|
|
|
|
|
|
|
|
|
|
40 |
FSH |
Male |
1.4-15.4IU/L |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 to 4 hours |
2 to 4 hour |
Gonadal function test |
Female |
|
Follicular Phase |
1-10IU/L |
Ovulatory Peak |
6-17IU/L |
Luteal Phase |
1-9IU/L |
Postmenopausal |
19-100IU/L |
41 |
LH |
Cord Blood |
0.04-2.6U/L |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2 to 4 hours |
2 to 4 hour |
Gonad dysfunction. Assessment of Infertility. |
Prepubertal child (2-11 months) |
0.02-8.0U/L |
1-10 Yrs |
0.08-3.9U/L |
Puberty |
0.04-15.0U/L |
Adult(Male) |
1.24-7.8U/L |
Women |
|
Follicular Phase |
1.68-15.0U/L |
Ovulatory Peak |
21.9-56.6U/L |
Luteal Phase |
0.61-16.3U/L |
Postmenopausal |
14.2-52.5U/L |
42 |
PTH |
|
10-65 pg/ml |
CMIA |
|
|
|
|
|
|
|
43 |
CORTISOL |
Cord Blood |
5-17 μg/dL |
CMIA |
Serum |
Sample to be collected at Morning 8 AM, and evening 4 PM. 8PM 3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 14 hours |
20 to 4hour |
Cushing syndrome. Addison’s disease and HRT |
Infant (1-7 days) |
2-11 μg/dL |
Child 1-16yrs 8am |
3-21 μg/dL |
Adult 8am |
5-23 μg/dL |
Adult 4pm |
3-16 μg/dL |
Adult 8pm |
<50% of 8am values |
44 |
BETA HCG |
Male & Non pregnant Female |
< 5.0 mIU/ml |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 to 4hours |
2to4 hour |
Pregnancy,Ectopicpregnancy, Choriocarcinoma |
Female (Pregnant) |
|
Weeks of gestation |
m IU/ml |
4 week |
5 - 100 |
5 week |
200 - 3000 |
6 week |
10000 -80000 |
7 - 14 week |
90000 - 500000 |
15 -26 week |
5000 - 80000 |
27-40 week |
3000 - 15000 |
|
TUMOR MARKERS |
|
|
|
|
|
|
|
|
|
|
45 |
AFP |
Cord blood |
<5000ng/ml |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24 * 7 |
Same day after 2-4 hours |
2 to 4 hour |
Marker of hepatocellular carcinoma and germ cell tumors Mention age, LMP and USG findings or clinical history. |
Pregnancy |
25-75ng/ml |
child upto 1yr |
<30ng/ml |
Adult |
<8.5ng/ml |
Early tumor marker |
10 -20 ng/ml |
Serum : Cancer |
>1000ng/ml |
46 |
CEA |
Non smokers |
<3ng/ml |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 to 4 hours |
2to 4 hour |
Colorectal adenocarcinoma |
Smokers |
<5ng/ml |
47 |
CA 19-9 |
|
<37U/mL |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 to 4hours |
2to4 hour |
Pancreatic malignancy |
48 |
TOTAL PSA |
Adult 40-49yrs |
0-2.5ng/mL |
CMIA |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hours |
2 to 4 hours |
To help detect and to monitor prostate cancer and BPH |
50-59yrs |
0-3.5ng/mL |
60-69yrs |
0-4.5ng/mL |
70-79yrs |
0-0.65ng/mL |
49 |
CA 125 |
Adult |
<35U/mL |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2to 4 hours |
2to4 hour |
Ovarian cancer marker |
|
TDM |
|
|
|
|
|
|
|
|
|
|
50 |
VALPROIC ACID ASSAY |
Therapeutic |
50-100 μg/mL |
CMIA |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hour |
2 to 4 hour |
to measure and monitor the amount of valproic acid present in the blood sample. |
Toxic |
>100 μg/mL |
|
IRON PROFILE |
|
|
|
|
|
|
|
|
|
|
51 |
FERRITIN |
Male |
12-300ng/ml |
CMIA |
Serum |
3 ml Blood in Plain tube |
Regular |
24*7 |
Same day after 2 to 4 hrs |
2 to 4 hour |
Iron deficiency and iron overload |
Female |
12-150ng/ml |
52 |
TIBC |
Infant |
100-400ug/dl |
Alumina adsorption |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hour |
2 to 4 hour |
measures the total capacity of your blood to bind and transport iron, and therefore reflects your body's iron stores. |
Thereafter |
250-450ug/dl |
53 |
Serum Iron |
|
31-144ug/dl |
Ferene |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hour |
2 to 4 hour |
To evaluate your body's current store of iron |
|
PANCREATIC PROFILE |
|
|
|
|
|
|
|
|
|
|
54 |
SERUM AMYLASE |
|
25-125 U/L |
CNPG3 substrate |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Diagnosis of Pancreatitis |
55 |
SERUM LIPASE |
|
<38 U/L |
Quinone dye |
Serum |
3 ml Blood in Plain tube |
Regular |
24 *7 |
Same day after 2hours |
1 hour |
Diagnosis of pancreatitis |
|
VITAMINS |
|
|
|
|
|
|
|
|
|
|
56 |
Vitamin D (25 OH) |
|
10-65ng/mL |
CMIA |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hours |
2 to 4 hours |
To investigate a problem related to bone metabolism or parathyroid function, possible vitamin D deficiency |
57 |
VITAMIN B12 |
Normal |
206-678 ng/L |
CMIA |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hours |
2 to 4 hour |
To help diagnose the cause of anaemia or neuropathy |
Acceptable |
>201 ng/L |
Deficiency |
<150 ng/L |
58 |
FOLATE |
|
2.6-12.2 μg/L |
CMIA |
serum |
2 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 hour |
2 to 4 hour |
To help diagnose the cause of anaemia or neuropathy |
|
CSF: CEREBROSPINAL FLUID |
|
|
|
|
|
|
|
|
|
|
59 |
SUGAR |
Infant/child |
60-80mg/dl |
Hexokinase |
CSF |
2 ml Fluid, No preservative |
Store in ice. Transport ASAP |
24*7 |
Same day after 2 hours |
1 hour |
Used for differentiating bacterial meningitis from others |
Adult |
40-70mg/dl |
60 |
PROTEIN |
Premature |
15-130mg/dl |
Micro protein |
CSF |
2 ml fluid, No preservative |
Transport ASAP |
24 *7 |
Same day after 2hours |
1 hour |
Differential diagnosis of meningitis |
Term Newborn |
40-120mg/dl |
< 1 month |
20-80mg/dl |
> 1month |
15-40mg/dl |
Ventricular fluid |
5-15mg/dl |
cisternal fluid |
15-25mg/dl |
61 |
CHLORIDE |
Infant |
110-130mmol/L |
ISE |
CSF |
2 ml Fluid, No preservative |
Store in ice. Transport ASAP |
24*7 |
Same day after 2 hours |
1 hour |
Used for differentiating bacterial meningitis from others |
Adult |
118-132mmol/L |
62 |
ANTI TPO |
|
0-5.61IU/ml |
CMIA |
Serum |
3 ml Blood in plain tube |
Regular |
24*7 |
Same day after 2 TO 4 hours |
2 to 4 hour |
Autoimmune thyroiditis |
63 |
BNP |
|
0-100pg/ml |
CMIA |
Plasma |
2ml Blood in EDTA tube |
R. Transport ASAP |
24*7 |
Same day after 2 hours |
1 hour |
Diagnosis of CHF, Exclusion of acute and non acute heart failure. |
64 |
hsCRP |
|
0-5mg/dl |
Immunoturbidimetric |
Serum |
3 ml Blood in Plain tube |
R |
24*7 |
Same day after 2hours |
1 hour |
Marker of inflammation |
65 |
D Dimer |
|
0-500ng/ml |
Immunoturbidimetry |
Plasma |
2 ml Blood in sodium citrate tube |
R Transport ASAP |
24*7 |
Same day after 2hours |
1 hour |
Exclusion of DVT and pulmonary embolism. |
|
|
For more information on tests look up in https://labtestsonline.org.uk/tests-index |
CMIA: Chemiluminiscence Microparticle Immuno Assay |
|